Purpose(Empty Backbone) Expresses the S. pyogenes sgRNA from the H1 promoter
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||53186||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
|Cloning Grade DNA||53186-DNA.cg||2 µg of cloning grade DNA in Tris buffer||1||$95|
This material is available to academics and nonprofits only.
- Backbone size (bp) 3451
Vector typeMammalian Expression, CRISPR
- Promoter human H1
Growth in Bacteria
- Cloning method Restriction Enzyme
- 3′ sequencing primer M13 Reverse (Common Sequencing Primers)
Information for Cloning Grade DNA (Catalog # 53186-DNA.cg) ( Back to top )
Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.
- Amount 2 µg
- Guaranteed Concentration 100 ng/µl +/- 5 ng/µl
- Pricing $95 USD
- Storage DNA can be stored at 4℃ (short term) or -20℃ (long term).
Terms and Licenses
- Not Available to Industry
Addgene has verified this plasmid using Next Generation Sequencing. Results are available here
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:phH1-gRNA was a gift from Charles Gersbach (Addgene plasmid # 53186 ; http://n2t.net/addgene:53186 ; RRID:Addgene_53186)
For your References section:Multiplex CRISPR/Cas9-based genome engineering from a single lentiviral vector. Kabadi AM, Ousterout DG, Hilton IB, Gersbach CA. Nucleic Acids Res. 2014 Aug 13. pii: gku749. 10.1093/nar/gku749 PubMed 25122746