pUAST tdTomato fascin WT
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||53508||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector typeInsect Expression
Growth in Bacteria
SpeciesD. melanogaster (fly)
Entrez Genesn (a.k.a. Dmel_CG32858, CG15331, CG1536, CG32858, Dmel\CG32858, SN, Sn, fs(1)A1057, fs(1)K1421, fs(1)K418, fs(1)K473, fs(1)K743, fs(1)M45)
- Promoter GAL4
/ Fusion Protein
- tdtomato (N terminal on backbone)
- Cloning method Gateway Cloning
- 5′ sequencing primer tdTomato-Fwd
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pUAST tdTomato fascin WT was a gift from Matthew Scott (Addgene plasmid # 53508 ; http://n2t.net/addgene:53508 ; RRID:Addgene_53508)
For your References section:Rab35 controls actin bundling by recruiting fascin as an effector protein. Zhang J, Fonovic M, Suyama K, Bogyo M, Scott MP. Science. 2009 Sep 4;325(5945):1250-4. doi: 10.1126/science.1174921. 10.1126/science.1174921 PubMed 19729655