PurposeNote- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 487, Emission: 509
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||54134||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4750
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Entrez GeneKRT18 (a.k.a. CK-18, CYK18, K18)
- Promoter CMV
/ Fusion Protein
- mEmerald (C terminal on backbone)
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
. Excitation = 487; Emission = 509. Localization data: Cytokeratin (18)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:mEmerald-Keratin-17 was a gift from Michael Davidson (Addgene plasmid # 54134 ; http://n2t.net/addgene:54134 ; RRID:Addgene_54134)
For your References section:Advances in fluorescent protein technology. Shaner NC, Patterson GH, Davidson MW. J Cell Sci. 2007 Dec 15;120(Pt 24):4247-60 doi: 10.1242/jcs.005801 PubMed 18057027