PurposeLocalization: Nucleus, Excitation: 487, Emission: 509
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||54174||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4750
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
SpeciesM. musculus (mouse)
- Promoter CMV
/ Fusion Protein
- mEmerald (C terminal on backbone)
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
. Excitation = 487; Emission = 509. Localization data: Nucleus
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:mEmerald-MCM7-N-22 was a gift from Michael Davidson (Addgene plasmid # 54174)
For your References section:Highly stable loading of Mcm proteins onto chromatin in living cells requires replication to unload. Kuipers MA, Stasevich TJ, Sasaki T, Wilson KA, Hazelwood KL, McNally JG, Davidson MW, Gilbert DM. J Cell Biol. 2011 Jan 10;192(1):29-41. doi: 10.1083/jcb.201007111. 10.1083/jcb.201007111 PubMed 21220507