Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Addgene is open for ordering and depositing; find up-to-date details here. To learn more about how we are supporting COVID-19 research and to find related plasmids, check out our COVID-19 and Coronavirus Plasmids & Resources page.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

(Plasmid #54221)


Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 54221 Standard format: Plasmid sent in bacteria as agar stab 1 $75 *

* Login to view industry pricing.


  • Vector backbone
  • Backbone size w/o insert (bp) 4750
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
    Human Proliferating Cell Nuclear Antigen
  • Alt name
  • Species
    H. sapiens (human)
  • Entrez Gene
    PCNA (a.k.a. ATLD2)
  • Promoter CMV
  • Tag / Fusion Protein
    • mEmerald (N terminal on backbone)

Cloning Information

Resource Information

Depositor Comments

Human Proliferating Cell Nuclear Antigen. Excitation = 487; Emission = 509. Localization data: Nucleus

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mEmerald-PCNA-19 was a gift from Michael Davidson (Addgene plasmid # 54221 ; ; RRID:Addgene_54221)
  • For your References section:

    G2 phase chromatin lacks determinants of replication timing. Lu J, Li F, Murphy CS, Davidson MW, Gilbert DM. J Cell Biol. 2010 Jun 14;189(6):967-80. doi: 10.1083/jcb.201002002. Epub 2010 Jun 7. 10.1083/jcb.201002002 PubMed 20530209