PurposeLocalization: Mitochondria, Excitation: 487, Emission: 509
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||54225||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4750
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Entrez GenePDHA1 (a.k.a. PDHA, PDHAD, PDHCE1A, PHE1A)
- Promoter CMV
/ Fusion Protein
- mEmerald (C terminal on backbone)
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
. Excitation = 487; Emission = 509. Localization data: Mitochondria
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:mEmerald-PDHA1-N-10 was a gift from Michael Davidson (Addgene plasmid # 54225)
For your References section:Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination. Planchon TA, Gao L, Milkie DE, Davidson MW, Galbraith JA, Galbraith CG, Betzig E. Nat Methods. 2011 May;8(5):417-23. doi: 10.1038/nmeth.1586. Epub 2011 Mar 4. 10.1038/nmeth.1586 PubMed 21378978