PurposeLocalization: Microtubules, Excitation: 487, Emission: 509
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||54292||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4750
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Entrez GeneTUBA1B (a.k.a. K-ALPHA-1)
- Promoter CMV
/ Fusion Protein
- mEmerald (N terminal on backbone)
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
. Excitation = 487; Emission = 509. Localization data: Microtubules
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:mEmerald-Tubulin-C-18 was a gift from Michael Davidson (Addgene plasmid # 54292 ; http://n2t.net/addgene:54292 ; RRID:Addgene_54292)
For your References section:Noninvasive imaging beyond the diffraction limit of 3D dynamics in thickly fluorescent specimens. Gao L, Shao L, Higgins CD, Poulton JS, Peifer M, Davidson MW, Wu X, Goldstein B, Betzig E. Cell. 2012 Dec 7;151(6):1370-85. doi: 10.1016/j.cell.2012.10.008. 10.1016/j.cell.2012.10.008 PubMed 23217717