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mApple-pBAD
(Plasmid #54536)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 54536 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    mApple-pBAD
  • Backbone size (bp) 4800
  • Vector type
    Bacterial Expression
  • Tags / Fusion Proteins
    • mApple (C terminal on backbone)
    • 6xHis (N terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None
  • Tags / Fusion Proteins
    • mApple (C terminal on backbone)
    • 6xHis (N terminal on backbone)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    N.C. Shaner, M.W. Davidson, and R.Y. Tsien; PMID 18454154
  • Terms and Licenses

Depositor Comments

Contains 6x-Histidine tag for protein purification. Excitation = 568; Emission = 592

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mApple-pBAD was a gift from Michael Davidson & Nathan Shaner & Roger Tsien (Addgene plasmid # 54536)
  • For your References section:

    Improving the photostability of bright monomeric orange and red fluorescent proteins. Shaner NC, Lin MZ, McKeown MR, Steinbach PA, Hazelwood KL, Davidson MW, Tsien RY. Nat Methods. 2008 Jun;5(6):545-51. doi: 10.1038/nmeth.1209. Epub 2008 May 4. 10.1038/nmeth.1209 PubMed 18454154