Purpose(Empty Backbone) Localization: N1 Cloning Vector, Excitation: 505, Emission: 515. This plasmid encodes dClover2 A206K, H231L based on publication.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||54538||Standard format: Plasmid sent in bacteria as agar stab||1||$75 *|
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- Backbone size (bp) 4750
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
/ Fusion Protein
- dClover2 (C terminal on backbone)
Growth in Bacteria
Copy numberHigh Copy
- Promoter CMV
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
. Excitation = 505; Emission = 515
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:dClover2-N1 was a gift from Michael Davidson (Addgene plasmid # 54538 ; http://n2t.net/addgene:54538 ; RRID:Addgene_54538)
For your References section:Improving brightness and photostability of green and red fluorescent proteins for live cell imaging and FRET reporting. Bajar BT, Wang ES, Lam AJ, Kim BB, Jacobs CL, Howe ES, Davidson MW, Lin MZ, Chu J. Sci Rep. 2016 Feb 16;6:20889. doi: 10.1038/srep20889. 10.1038/srep20889 PubMed 26879144