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mPlum-pBAD
(Plasmid #54564)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 54564 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    mPlum-pBAD
  • Backbone size (bp) 4800
  • Vector type
    Bacterial Expression
  • Tags / Fusion Proteins
    • mPlum (N terminal on backbone)
    • 6xHis (N terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None
  • Tags / Fusion Proteins
    • mPlum (N terminal on backbone)
    • 6xHis (N terminal on backbone)

Resource Information

Depositor Comments

Contains 6x-Histidine tag for protein purification. Excitation = 590; Emission = 649

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mPlum-pBAD was a gift from Michael Davidson & Roger Tsien (Addgene plasmid # 54564)
  • For your References section:

    Evolution of new nonantibody proteins via iterative somatic hypermutation. Wang L, Jackson WC, Steinbach PA, Tsien RY. Proc Natl Acad Sci U S A. 2004 Nov 30;101(48):16745-9. Epub 2004 Nov 19. 10.1073/pnas.0407752101 PubMed 15556995