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YPet-N1
(Plasmid #54637)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 54637 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    YPet-N1
  • Backbone size (bp) 4750
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)
  • Tag / Fusion Protein
    • YPet (C terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None
  • Tag / Fusion Protein
    • YPet (C terminal on backbone)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    P.S. Daugherty, PMID 15696158
  • Terms and Licenses

Depositor Comments

. Excitation = 513; Emission = 527

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    YPet-N1 was a gift from Patrick Daugherty & Michael Davidson (Addgene plasmid # 54637)
  • For your References section:

    Evolutionary optimization of fluorescent proteins for intracellular FRET. Nguyen AW, Daugherty PS. Nat Biotechnol. 2005 Mar . 23(3):355-60. 10.1038/nbt1066 PubMed 15696158