|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||55927||Standard format: Plasmid sent in bacteria as agar stab||1||$75 *|
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- Backbone size w/o insert (bp) 4750
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Entrez GeneACTN1 (a.k.a. BDPLT15)
- Promoter CMV
/ Fusion Protein
- JRed (C terminal on insert)
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
. Excitation = 584; Emission = 610
There's an EGFP directly downstream of JRed however it is not in frame with Alpha Actinin or JRed.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:JRed-Alpha-Actinin-19 was a gift from Michael Davidson (Addgene plasmid # 55927 ; http://n2t.net/addgene:55927 ; RRID:Addgene_55927)