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mIFP12-H2B-C-10
(Plasmid #56254)

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Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Plasmid 56254 Standard format: Plasmid sent in bacteria as agar stab 1 $75 *

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Backbone

  • Vector backbone
    mIFP12-C1
  • Backbone size w/o insert (bp) 4750
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    H2B
  • Alt name
    Human Histone H2B
  • Alt name
    NM_021058.3
  • Species
    H. sapiens (human)
  • Mutation
    D26G and V119I in H2B
  • Entrez Gene
    H2BC11 (a.k.a. H2B/r, H2BFR, H2BJ, HIST1H2BJ)
  • Promoter CMV
  • Tag / Fusion Protein
    • mIFP12 (N terminal on backbone)

Cloning Information

Resource Information

Depositor Comments

H. sapiens (human) Histone H2B. Excitation = 683; Emission = 704

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mIFP12-H2B-C-10 was a gift from Michael Davidson (Addgene plasmid # 56254 ; http://n2t.net/addgene:56254 ; RRID:Addgene_56254)
  • For your References section:

    A naturally monomeric infrared fluorescent protein for protein labeling in vivo. Yu D, Baird MA, Allen JR, Howe ES, Klassen MP, Reade A, Makhijani K, Song Y, Liu S, Murthy Z, Zhang SQ, Weiner OD, Kornberg TB, Jan YN, Davidson MW, Shu X. Nat Methods. 2015 Aug;12(8):763-765. doi: 10.1038/nmeth.3447. Epub 2015 Jun 22. 10.1038/nmeth.3447 PubMed 26098020