Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.
Holiday Schedule: Addgene will be closed November 28th & 29th for the Thanksgiving holiday. Order processing and shipping may be delayed the week of November 25th - 29th. If you have any questions, please contact us at [email protected].

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pAAV/D374Y-hPCSK9
(Plasmid #58379)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 58379 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAAV/mU6.siRNA.shuttle
  • Backbone manufacturer
    Applied Viromics, Fremont, CA, USA
  • Total vector size (bp) 6518
  • Modifications to backbone
    mU6.siRNA cassette removed
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    proprotein convertase subtilisin/kexin type 9
  • Alt name
    PCSK9
  • Alt name
    NARC1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2078
  • Mutation
    D374Y
  • Entrez Gene
    PCSK9 (a.k.a. FH3, FHCL3, HCHOLA3, LDLCQ1, NARC-1, NARC1, PC9)
  • Promoter HCRApoE/hAAT

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (not destroyed)
  • 3′ cloning site FseI (not destroyed)
  • 5′ sequencing primer na
  • 3′ sequencing primer na
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    PCR amplified from human cDNA clone (SC101299, OriGene)
  • Terms and Licenses

Depositor Comments

Insert contains G670E and V474I, which are known SNPs in the human PCSK9 gene,

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV/D374Y-hPCSK9 was a gift from Jacob Bentzon (Addgene plasmid # 58379 ; http://n2t.net/addgene:58379 ; RRID:Addgene_58379)
  • For your References section:

    Induction of atherosclerosis in mice and hamsters without germline genetic engineering. Bjorklund MM, Hollensen AK, Hagensen MK, Dagnaes-Hansen F, Christoffersen C, Mikkelsen JG, Bentzon JF. Circ Res. 2014 May 23;114(11):1684-9. doi: 10.1161/CIRCRESAHA.114.302937. Epub 2014 Mar 27. 10.1161/CIRCRESAHA.114.302937 PubMed 24677271