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pAAV/D374Y-hPCSK9
(Plasmid #58379)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 58379 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAAV/mU6.siRNA.shuttle
  • Backbone manufacturer
    Applied Viromics, Fremont, CA, USA
  • Total vector size (bp) 6518
  • Modifications to backbone
    mU6.siRNA cassette removed
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    proprotein convertase subtilisin/kexin type 9
  • Alt name
    PCSK9
  • Alt name
    NARC1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2078
  • Mutation
    D374Y
  • Entrez Gene
    PCSK9 (a.k.a. FH3, FHCL3, HCHOLA3, LDLCQ1, NARC-1, NARC1, PC9)
  • Promoter HCRApoE/hAAT

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (not destroyed)
  • 3′ cloning site FseI (not destroyed)
  • 5′ sequencing primer na
  • 3′ sequencing primer na
  • (Common Sequencing Primers)

Resource Information

  • Addgene Notes
  • A portion of this plasmid was derived from a plasmid made by
    PCR amplified from human cDNA clone (SC101299, OriGene)
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Insert contains G670E and V474I, which are known SNPs in the human PCSK9 gene,

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV/D374Y-hPCSK9 was a gift from Jacob Bentzon (Addgene plasmid # 58379 ; http://n2t.net/addgene:58379 ; RRID:Addgene_58379)
  • For your References section:

    Induction of atherosclerosis in mice and hamsters without germline genetic engineering. Bjorklund MM, Hollensen AK, Hagensen MK, Dagnaes-Hansen F, Christoffersen C, Mikkelsen JG, Bentzon JF. Circ Res. 2014 May 23;114(11):1684-9. doi: 10.1161/CIRCRESAHA.114.302937. Epub 2014 Mar 27. 10.1161/CIRCRESAHA.114.302937 PubMed 24677271