PurposeExpress FLAG-tagged Luciferase in mammalian cells
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||58792||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Growth Strain(s)XL1 Blue
Copy numberHigh Copy
Gene/Insert namefirefly Luciferase
Insert Size (bp)1650
- Promoter CMV
/ Fusion Protein
- FLAG (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA3-FLAG-Luciferase was a gift from Hiroyoshi Ariga (Addgene plasmid # 58792 ; http://n2t.net/addgene:58792 ; RRID:Addgene_58792)
For your References section:A split luciferase-based reporter for detection of a cellular macromolecular complex. Maita H, Tomita K, Ariga H. Anal Biochem. 2014 Feb 3. pii: S0003-2697(14)00035-9. doi: 10.1016/j.ab.2014.01.015. 10.1016/j.ab.2014.01.015 PubMed 24503441