PurposeFor use in trypanosome cell lines already expressing T7 polymerase and Tet-repressor. Tetracycline induction results in the production of dsRNA targeting VSG221.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||59730||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
- Total vector size (bp) 2900
Growth in Bacteria
Gene/Insert nameVSG221 RNAi fragment
SpeciesT. brucei Lister 427
Insert Size (bp)803
- Promoter opposing T7 promoters
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (unknown if destroyed)
- 3′ cloning site XhoI (unknown if destroyed)
- 5′ sequencing primer atacagaatgtctttggc (Common Sequencing Primers)
Please note that Addgene QC did not confirm the RNAi fragment, but the linear size of the digested vector give the predicted result.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:MC177VSG221RNAi was a gift from Gloria Rudenko (Addgene plasmid # 59730)
For your References section:Variant surface glycoprotein RNA interference triggers a precytokinesis cell cycle arrest in African trypanosomes. Sheader K, Vaughan S, Minchin J, Hughes K, Gull K, Rudenko G. Proc Natl Acad Sci U S A. 2005 Jun 14;102(24):8716-21. Epub 2005 Jun 3. 10.1073/pnas.0501886102 PubMed 15937117