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MC177VSG221RNAi
(Plasmid #59730)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 59730 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBluescriptII
  • Total vector size (bp) 2900
  • Vector type
    RNAi
  • Selectable markers
    Phleomycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    VSG221 RNAi fragment
  • Species
    T. brucei Lister 427
  • Insert Size (bp)
    803
  • Promoter opposing T7 promoters

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer atacagaatgtctttggc
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Please note that Addgene QC did not confirm the RNAi fragment, but the linear size of the digested vector give the predicted result.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MC177VSG221RNAi was a gift from Gloria Rudenko (Addgene plasmid # 59730)
  • For your References section:

    Variant surface glycoprotein RNA interference triggers a precytokinesis cell cycle arrest in African trypanosomes. Sheader K, Vaughan S, Minchin J, Hughes K, Gull K, Rudenko G. Proc Natl Acad Sci U S A. 2005 Jun 14;102(24):8716-21. Epub 2005 Jun 3. 10.1073/pnas.0501886102 PubMed 15937117