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tol2-CAG::ChR2-YFP
(Plasmid #59740)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 59740 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pUC18
  • Modifications to backbone
    tol2L/R flanked expression vector consisting of CAG promoter, followed by a 5' splice substrate, the ChR2-YFP gene, a bGH poly-adenylation sequence, and a FRT-zeo-FRT resistance marker.
  • Vector type
    Mammalian Expression
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Channelrhodopsin-2
  • Alt name
    ChR2
  • Species
    Chlamydomonas reinhardtii
  • Mutation
    H134R
  • Promoter CAG
  • Tag / Fusion Protein
    • YFP (C terminal on insert)

Cloning Information

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    tol2-CAG::ChR2-YFP was a gift from Ivo Lieberam (Addgene plasmid # 59740)
  • For your References section:

    Optical control of muscle function by transplantation of stem cell-derived motor neurons in mice. Bryson JB, Machado CB, Crossley M, Stevenson D, Bros-Facer V, Burrone J, Greensmith L, Lieberam I. Science. 2014 Apr 4;344(6179):94-7. doi: 10.1126/science.1248523. 10.1126/science.1248523 PubMed 24700859