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pAAV-myrSNAP
(Plasmid #60098)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 60098 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAAV-CMV
  • Backbone manufacturer
    custom
  • Backbone size w/o insert (bp) 4600
  • Total vector size (bp) 6600
  • Vector type
    Mammalian Expression, Mouse Targeting, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    SNAP-tag
  • Species
    Synthetic
  • Insert Size (bp)
    546

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer SNAPfw
  • 3′ sequencing primer SNAPrev
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    histone-GFP
  • Species
    Synthetic
  • Insert Size (bp)
    1220

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer EGFPfw
  • 3′ sequencing primer EGFPrev
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-myrSNAP was a gift from Gregory Jefferis (Addgene plasmid # 60098 ; http://n2t.net/addgene:60098 ; RRID:Addgene_60098)
  • For your References section:

    Ultrafast tissue staining with chemical tags. Kohl J, Ng J, Cachero S, Ciabatti E, Dolan MJ, Sutcliffe B, Tozer A, Ruehle S, Krueger D, Frechter S, Branco T, Tripodi M, Jefferis GS. Proc Natl Acad Sci U S A. 2014 Aug 25. pii: 201411087. 10.1073/pnas.1411087111 PubMed 25157152