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psAAVS1-T2
(Plasmid #60543)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 60543 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP C1
  • Backbone manufacturer
    ClonTech
  • Backbone size w/o insert (bp) 4685
  • Total vector size (bp) 4731
  • Modifications to backbone
    The BsaI site in the backbone was removed by site directed mutagenesis (C3755T).
  • Vector type
    Mammalian Expression, CRISPR ; Kan

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ProtoSpacer AAVS1-T2
  • Alt name
    psAAVS1-T2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    46

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsaI (unknown if destroyed)
  • 3′ cloning site BsaI (unknown if destroyed)
  • 5′ sequencing primer CCA TTGACGTCAA TGGGAG
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    psAAVS1-T2 was a gift from William Simonds (Addgene plasmid # 60543 ; http://n2t.net/addgene:60543 ; RRID:Addgene_60543)
  • For your References section:

    Improving the specificity and efficacy of CRISPR/CAS9 and gRNA through target specific DNA reporter. Zhang JH, Pandey M, Kahler JF, Loshakov A, Harris B, Dagur PK, Mo Y, Simonds WF. J Biotechnol. 2014 Sep 2. pii: S0168-1656(14)00838-4. doi: 10.1016/j.jbiotec.2014.08.033. 10.1016/j.jbiotec.2014.08.033 PubMed 25193712