pBUN276
(Plasmid
#60673)
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Purposeoverexpression of M. tuberculosis ddl gene in Mycobacterium
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 60673 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepMV262
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Backbone manufacturerMedImmune Inc.
- Total vector size (bp) 5600
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Vector typeE.coli-Mycobacterium shuttle vector
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameddl
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SpeciesM. tuberculosis
- Promoter BCG Hsp60
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Tag
/ Fusion Protein
- 6 codons of M. bovis BCG hsp60 (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (unknown if destroyed)
- 3′ cloning site HindIII (unknown if destroyed)
- 5′ sequencing primer unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBUN276 was a gift from Raul Barletta (Addgene plasmid # 60673 ; http://n2t.net/addgene:60673 ; RRID:Addgene_60673) -
For your References section:
Roles of Mycobacterium smegmatis D-alanine:D-alanine ligase and D-alanine racemase in the mechanisms of action of and resistance to the peptidoglycan inhibitor D-cycloserine. Feng Z, Barletta RG. Antimicrob Agents Chemother. 2003 Jan;47(1):283-91. PubMed 12499203
Map uploaded by the depositor.
