pTriEx-mCherry::LANS1
(Plasmid #60784)

• Purpose: Control of Protein Activity and Cell Fate Specification via Light-Mediated Nuclear Translocation
• Depositing Lab: Brian Kuhlman
• Publication: Control of cell fate specification in vivo via light-conditioned nuclear translocation (unpublished)
• Sequence Information:

  • Depositor Sequences: Full (1) Partial (1)
  • Addgene Sequences: Partial (2)

Ordering

Item	Catalog #	Description	Quantity	Price (USD)
Plasmid	60784	Plasmid sent as bacteria in agar stab	1	$65	Add to Cart

This material is available to academics and nonprofits only.

Backbone

• Vector backbone: pTriEx
  (Search Vector Database)
• Backbone size w/o insert (bp): 5943
• Total vector size (bp): 6429
• Vector type: Mammalian Expression, Bacterial Expression, Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: LANS1
• Species: Synthetic; Avena Sativa
• Promoter: CMV
• Tag / Fusion Protein:
  • mCherry (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: CCCAACACAATATATTATAGTTAAATAAGAATTATTATC
• 3′ sequencing primer: GGTGGTGCTCGAGATCCTCGGGG

Resource Information

• Supplemental Documents:
  • LANS1
• Terms and Licenses:
  • UBMTA
  • Clontech Limited Use Label License
  • EMD Millipore Limited Use Label License pTriEx Assurance Letter
• Article Citing this Plasmid:
  • 1 Reference