pTriEx-mCherry::LANS1
(Plasmid
#60784)
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PurposeControl of Protein Activity and Cell Fate Specification via Light-Mediated Nuclear Translocation
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | ||
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Plasmid | 60784 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $75 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepTriEx
- Backbone size w/o insert (bp) 5943
- Total vector size (bp) 6429
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Vector typeMammalian Expression, Bacterial Expression, Insect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLANS1
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SpeciesSynthetic; Avena Sativa
- Promoter CMV
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Tag
/ Fusion Protein
- mCherry (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer CCCAACACAATATATTATAGTTAAATAAGAATTATTATC
- 3′ sequencing primer GGTGGTGCTCGAGATCCTCGGGG (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Terms and Licenses
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Industry Terms
- Not Available to Industry
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Article Citing this Plasmid
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTriEx-mCherry::LANS1 was a gift from Brian Kuhlman (Addgene plasmid # 60784 ; http://n2t.net/addgene:60784 ; RRID:Addgene_60784)