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pcDNA-dCas9-p300 Core (H1415A/E1423A/Y1424A/L1428S/Y1430A/H1434A)
(Plasmid #61364)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 61364 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen; Life Technologies
  • Total vector size (bp) 11475
  • Vector type
    Mammalian Expression, CRISPR, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300)
  • Species
    H. sapiens (human), Synthetic; S. pyogenes
  • Insert Size (bp)
    6138
  • Mutation
    D10A; H840A in S.pyogenes Cas9; H1415A/E1423A/Y1424A/L1428S/Y1430A/H1434A mutation in p300 Core
  • GenBank ID
    WP_010922251.1 NP_001420.2
  • Entrez Gene
    EP300 (a.k.a. KAT3B, RSTS2, p300)
  • Promoter CMV
  • Tags / Fusion Proteins
    • Flag (N terminal on insert)
    • SV40 NLS (N terminal on insert)
    • SV40 NLS (C terminal on insert)
    • HA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AscI (not destroyed)
  • 3′ cloning site PmeI (not destroyed)
  • 5′ sequencing primer CMV F
  • 3′ sequencing primer Bgh R
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA-dCas9-p300 Core (H1415A/E1423A/Y1424A/L1428S/Y1430A/H1434A) was a gift from Charles Gersbach (Addgene plasmid # 61364)
  • For your References section:

    Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers. Hilton IB, D'Ippolito AM, Vockley CM, Thakore PI, Crawford GE, Reddy TE, Gersbach CA. Nat Biotechnol. 2015 May;33(5):510-7. doi: 10.1038/nbt.3199. Epub 2015 Apr 6. 10.1038/nbt.3199 PubMed 25849900