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Purpose3rd generation lentiviral vector; TetON promoter driving human Ngn1; constitutive expression of Blasticidine selection marker
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 61473 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepFUGW
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Backbone manufacturerLab of David Baltimore
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Vector typeLentiviral
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert nameNgn1
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Alt nameNeurogenin1
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SpeciesH. sapiens (human)
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Entrez GeneNEUROG1 (a.k.a. AKA, Math4C, NEUROD3, bHLHa6, ngn1)
Gene/Insert 2
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Gene/Insert nameBla
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Alt nameBlasticidin
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLV_TRET_hNgn1_UBC_Bla was a gift from Ron Weiss (Addgene plasmid # 61473 ; http://n2t.net/addgene:61473 ; RRID:Addgene_61473) -
For your References section:
Rapid neurogenesis through transcriptional activation in human stem cells. Busskamp V, Lewis NE, Guye P, Ng AH, Shipman SL, Byrne SM, Sanjana NE, Murn J, Li Y, Li S, Stadler M, Weiss R, Church GM. Mol Syst Biol. 2014 Nov 17;10(11):760. doi: 10.15252/msb.20145508. PubMed 25403753
Map uploaded by the depositor.
