pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA
(Plasmid #61591)

• Purpose: A single vector AAV-Cas9 system containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA.
• Depositing Lab: Feng Zhang
• Publication: Ran et al Nature. 2015 Apr 1. doi: 10.1038/nature14299.
• Sequence Information:

  • Depositor Sequences: None.
  • Addgene Sequences: Full (1) Partial (4)

Ordering

Item	Catalog #	Description	Quantity	Price (USD)
Plasmid	61591	Plasmid sent as bacteria in agar stab	1	$65	Add to Cart

This material is available to academics and nonprofits only.

Backbone

• Vector backbone: pAAV
  (Search Vector Database)
• Vector type: Mammalian Expression, AAV, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: hSaCas9
• Species: Staphylococcus aureus
• Insert Size (bp): 3345
• GenBank ID: CCK74173.1
• Tags / Fusion Proteins:
  • NLS (N terminal on insert)
  • NLS (C terminal on insert)
  • 3xHA (C terminal on insert)

Gene/Insert 2

• Gene/Insert name: Chimeric guide for SaCas9
• Species: Staphylococcus aureus
• Insert Size (bp): 101

Resource Information

• Supplemental Documents:
  • pX601-CMV-SaCas9(reopt)-3xHA-U6-BsaI(sgRNA).gbk
  • SaCas9 User Manual
• Addgene Notes:
  • Addgene Diagnostic Digest
• Terms and Licenses:
  • UBMTA
• Articles Citing this Plasmid:
  • 5 References