Purpose(Empty Backbone) UAS transgenesis vector
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||62215||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 2644
Vector typeZebrafish transgenesis
- Promoter UAS-E1b
Growth in Bacteria
Copy numberHigh Copy
Transgenesis vector with tol1 arms, 14xUAS-E1b promoter, ubiquitin C intron, multiple cloning site and ocean pout antifreeze protein terminator. Co-inject with tol1 (not tol2) transposase.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pT1UciMP was a gift from Harold Burgess (Addgene plasmid # 62215 ; http://n2t.net/addgene:62215 ; RRID:Addgene_62215)
For your References section:Increased functional protein expression using nucleotide sequence features enriched in highly expressed genes in zebrafish. Horstick EJ, Jordan DC, Bergeron SA, Tabor KM, Serpe M, Feldman B, Burgess HA. Nucleic Acids Res. 2015 Jan 27. pii: gkv035. 10.1093/nar/gkv035 PubMed 25628360