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Addgene

pMF230
(Plasmid #62546)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 62546 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pMF36
  • Backbone manufacturer
    PMID 8394313
  • Modifications to backbone
    pKK233-2 with ori-T and stabilization fragment.
  • Vector type
    Broad host range plasmid

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    GFP mut2
  • Species
    Synthetic
  • Promoter trc

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer M13pUC-Rev
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMF230 was a gift from Michael Franklin (Addgene plasmid # 62546 ; http://n2t.net/addgene:62546 ; RRID:Addgene_62546)
  • For your References section:

    Role of alginate and its O acetylation in formation of Pseudomonas aeruginosa microcolonies and biofilms. Nivens DE, Ohman DE, Williams J, Franklin MJ. J Bacteriol. 2001 Feb;183(3):1047-57. 10.1128/JB.183.3.1047-1057.2001 PubMed 11208804