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PurposeBroad host range plasmid for constitutive expression of the eGFP. Developed for imaging Pseudomonas aeruginosa in biofilms.
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 62546 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMF36
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Backbone manufacturerPMID 8394313
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Modifications to backbonepKK233-2 with ori-T and stabilization fragment.
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Vector typeBroad host range plasmid
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameGFP mut2
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SpeciesSynthetic
- Promoter trc
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer M13pUC-Rev (Common Sequencing Primers)
Resource Information
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Addgene Notes
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMF230 was a gift from Michael Franklin (Addgene plasmid # 62546 ; http://n2t.net/addgene:62546 ; RRID:Addgene_62546) -
For your References section:
Role of alginate and its O acetylation in formation of Pseudomonas aeruginosa microcolonies and biofilms. Nivens DE, Ohman DE, Williams J, Franklin MJ. J Bacteriol. 2001 Feb;183(3):1047-57. 10.1128/JB.183.3.1047-1057.2001 PubMed 11208804