Purpose(Empty Backbone) TALEN array receiving vector with half-site HD RVD
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||62591||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 6840
Modifications to backboneLacZ screening cassette flanked by BbsI sites for golden gate cloning
Vector typeMammalian Expression, TALEN ; T7
- Promoter CMV
/ Fusion Proteins
- 3× FLAG (N terminal on backbone)
- FOKI WT (C terminal on backbone)
Growth in Bacteria
Copy numberHigh Copy
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byThis material was constructed from JDS71 (AddGene Plasmid #32287). The TALEN insertion site was replaced with a LacZ selection cassette flanked by two BbsI sites. Other BbsI sites in the plasmid backbone were mutated.
Terms and Licenses
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:AI-CN442 was a gift from Joshua Grimley (Addgene plasmid # 62591 ; http://n2t.net/addgene:62591 ; RRID:Addgene_62591)
For your References section:Genome engineering of isogenic human ES cells to model autism disorders. Martinez RA, Stein JL, Krostag AR, Nelson AM, Marken JS, Menon V, May RC, Yao Z, Kaykas A, Geschwind DH, Grimley JS. Nucleic Acids Res. 2015 Mar 11. pii: gkv164. 10.1093/nar/gkv164 PubMed 25765640