This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pET-NLS-Cas9-6xHis
(Plasmid #62934)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 62934 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET29
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5161
  • Total vector size (bp) 9295
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Mach1
  • Growth instructions
    For protein expression, transform into BL21 Star (DE3) cells. Induce at 0.6 OD with 0.5 mM IPTG final concentration. Growing overnight (16 hours) at 20-25 C.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NLS-Cas9-6xHis
  • Species
    S. pyogenes
  • Insert Size (bp)
    4152
  • Promoter T7
  • Tags / Fusion Proteins
    • 6xHis tag (C terminal on backbone)
    • NLS (N terminal on insert)

Cloning Information

Resource Information

Depositor Comments

Alternative plasmid name: pJAZ0006

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-NLS-Cas9-6xHis was a gift from David Liu (Addgene plasmid # 62934)
  • For your References section:

    Cationic lipid-mediated delivery of proteins enables efficient protein-based genome editing in vitro and in vivo. Zuris JA, Thompson DB, Shu Y, Guilinger JP, Bessen JL, Hu JH, Maeder ML, Joung JK, Chen ZY, Liu DR. Nat Biotechnol. 2015 Jan;33(1):73-80. doi: 10.1038/nbt.3081. Epub 2014 Oct 30. 10.1038/nbt.3081 PubMed 25357182