|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||63121||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Modifications to backboneFRT-flanked GmR marker was inserted within miniTn7 element
Vector typeBacterial Expression
Growth in Bacteria
Growth instructionsalso ampicillin resistant
Copy numberHigh Copy
- Cloning method Restriction Enzyme
Terms and Licenses
- Not Available to Industry
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pUC18T-mini-Tn7T-Gm was a gift from Herbert Schweizer (Addgene plasmid # 63121 ; http://n2t.net/addgene:63121 ; RRID:Addgene_63121)
For your References section:A Tn7-based broad-range bacterial cloning and expression system. Choi KH, Gaynor JB, White KG, Lopez C, Bosio CM, Karkhoff-Schweizer RR, Schweizer HP. Nat Methods. 2005 Jun;2(6):443-8. 10.1038/nmeth765 PubMed 15908923