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Addgene

pDestTol2CG2-U6:gRNA Notes

Addgene Diagnostic Digest

This gel depicts the NEB 1kb ladder and a digestion of the plasmid pDestTol2CG2-U6:gRNA with the following enzymes: SpeI-HF and XhoI.

We expect and see bands at 3.4, 2.7, and 2.2 kb.

Addgene Diagnostic Digest

This gel depicts the NEB 1kb ladder and a digestion of the plasmid
pDestTol2CG2-U6:gRNA with the following enzymes: EcoRI-HF
and Sac II.

Expected results:
EcoRI-HF/Sac II 4659 + 3601 bp

We observed the expected bands.