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Or46a-Gal4
(Plasmid #63179)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 63179 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCasperAUG-VP22-Gal4
  • Backbone manufacturer
    Leslie Vosshall
  • Backbone size w/o insert (bp) 10000
  • Total vector size (bp) 11888
  • Modifications to backbone
    This vector contains a fusion of the first ~30 amino acids of Drosophila melanogaster alcohol dehydrogenase gene, followed by the Herpes simplex virus 1 (HSV-1) protein VP22, encoded by the UL49 gene, followed by the S. cerevisiae (budding yeast) Gal4 gene. This translational fusion generates a single protein: AUG-Adh-VP22-Gal4
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Or46a promoter
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    1888

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pCasper-F 5'-gggttttattaacttacat-3'
  • 3′ sequencing primer GAL4Nterm-R 5'-tcagatgtgccctagtcagc-3'
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

Or46a-VP22-Gal4 consists of the 1.888 Kb Drosophila melanogaster Or46a promoter subcloned into the EcoRI site of the pCaSpeR-AUG-VP22-Gal4 vector. This VP22-Gal4 is a fusion of the HSV-1 structural protein VP22 (Elliott and O’Hare, 1997; PMID: 9008163) to yeast Gal4. The activity of this fusion protein is indistinguishable from that of unmodified Gal4 and did not show the intercellular protein transfer properties of unmodified VP22 (Elliott and O’Hare, 1997; PMID: 9008163).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Or46a-Gal4 was a gift from Leslie Vosshall (Addgene plasmid # 63179)
  • For your References section:

    Genetic and functional subdivision of the Drosophila antennal lobe. Fishilevich E, Vosshall LB. Curr Biol. 2005 Sep 6;15(17):1548-53. 10.1016/j.cub.2005.07.066 PubMed 16139209