PurposeExpresses Gal4 under control of the Drosophila melanogaster Or46a odorant receptor promoter
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||63179||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerLeslie Vosshall
- Backbone size w/o insert (bp) 10000
- Total vector size (bp) 11888
Modifications to backboneThis vector contains a fusion of the first ~30 amino acids of Drosophila melanogaster alcohol dehydrogenase gene, followed by the Herpes simplex virus 1 (HSV-1) protein VP22, encoded by the UL49 gene, followed by the S. cerevisiae (budding yeast) Gal4 gene. This translational fusion generates a single protein: AUG-Adh-VP22-Gal4
Vector typeInsect Expression
Growth in Bacteria
Gene/Insert nameOr46a promoter
SpeciesD. melanogaster (fly)
Insert Size (bp)1888
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer pCasper-F 5'-gggttttattaacttacat-3'
- 3′ sequencing primer GAL4Nterm-R 5'-tcagatgtgccctagtcagc-3' (Common Sequencing Primers)
Terms and Licenses
Or46a-VP22-Gal4 consists of the 1.888 Kb Drosophila melanogaster Or46a promoter subcloned into the EcoRI site of the pCaSpeR-AUG-VP22-Gal4 vector. This VP22-Gal4 is a fusion of the HSV-1 structural protein VP22 (Elliott and O’Hare, 1997; PMID: 9008163) to yeast Gal4. The activity of this fusion protein is indistinguishable from that of unmodified Gal4 and did not show the intercellular protein transfer properties of unmodified VP22 (Elliott and O’Hare, 1997; PMID: 9008163).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Or46a-Gal4 was a gift from Leslie Vosshall (Addgene plasmid # 63179)
For your References section:Genetic and functional subdivision of the Drosophila antennal lobe. Fishilevich E, Vosshall LB. Curr Biol. 2005 Sep 6;15(17):1548-53. 10.1016/j.cub.2005.07.066 PubMed 16139209
Map uploaded by the depositor.