Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

p415GPD-3xHA-GW
(Plasmid #63781)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 63781 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    p415GPD
  • Backbone manufacturer
    Mumberg D, et al. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene 15
  • Backbone size (bp) 8652
  • Modifications to backbone
    Added 3xHA tag and gateway converted
  • Vector type
    Yeast Expression
  • Promoter GPD
  • Selectable markers
    LEU2
  • Tag / Fusion Protein
    • 3xHA (N terminal on insert)

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DB3.1
  • Copy number
    Unknown

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

  • Terms and Licenses

Depositor Comments

Plasmid a modified form of p415GPD from:
Mumberg D, et al. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene 156: 119-122, 1995. PubMed: 7737504

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p415GPD-3xHA-GW was a gift from Susan Baserga (Addgene plasmid # 63781 ; http://n2t.net/addgene:63781 ; RRID:Addgene_63781)
  • For your References section:

    A protein interaction map of the LSU processome. McCann KL, Charette JM, Vincent NG, Baserga SJ. Genes Dev. 2015 Apr 15;29(8):862-75. doi: 10.1101/gad.256370.114. Epub 2015 Apr 15. 10.1101/gad.256370.114 PubMed 25877921