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pSG 25
(Plasmid #63867)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 63867 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Tetracycline
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    lacZ
  • Species
    Escherichia coli
  • Insert Size (bp)
    3200
  • GenBank ID
    NC_000913.3
  • Entrez Gene
    lacZ (a.k.a. b0344, ECK0341, JW0335)
  • Promoter PtacI

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NaeI (destroyed during cloning)
  • 3′ cloning site ScaI (destroyed during cloning)
  • 5′ sequencing primer M13pUC-Rev
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The lacZ gene originated from pKK-CUG (Bob Weiss; Wagner et al. 1994 J. Bact. 176:1683)
  • Terms and Licenses

Depositor Comments

pSG25 expresses very high levels of beta-galactosidase in E. coli. Various mutant derivatives are used to measure translational errors.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSG 25 was a gift from Albert Dahlberg (Addgene plasmid # 63867 ; http://n2t.net/addgene:63867 ; RRID:Addgene_63867)
  • For your References section:

    Mutations at U2555, a tRNA-protected base in 23S rRNA, affect translational fidelity. O'Connor M, Dahlberg AE. Proc Natl Acad Sci U S A. 1993 Oct 1;90(19):9214-8. PubMed 8415679