|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||63934||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
/ Fusion Protein
- Glycine-Serine-3XFlag-P2A-NeoR (C terminal on backbone)
Growth in Bacteria
- 5′ sequencing primer cagcaggctgaagttagtagc
- 3′ sequencing primer cttctatcgccttcttgacgag (Common Sequencing Primers)
Note: This plasmid is part of the Mendenhall and Meyers CRISPR-based Tagging system to add a tag (currently using FLAG) to endogenous proteins. Please see https://www.addgene.org/crispr/tagging/ for more details.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pFETCh_Donor (EMM0021) was a gift from Eric Mendenhall & Richard M. Myers (Addgene plasmid # 63934 ; http://n2t.net/addgene:63934 ; RRID:Addgene_63934)
For your References section:CETCh-seq: CRISPR epitope tagging ChIP-seq of DNA-binding proteins. Savic D, Partridge EC, Newberry KM, Smith SB, Meadows SK, Roberts BS, Mackiewicz M, Mendenhall EM, Myers RM. Genome Res. 2015 Sep 9. 10.1101/gr.193540.115 PubMed 26355004