PurposeMammalian expression of paired domain of Pax8 fused to the N-terminal repressor domain of the drosophila engrailed protein (Eng)
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||64039||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5446
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namePax8 paired domain
Alt namepaired box 8
SpeciesM. musculus (mouse)
Mutationpaired domain, aa 1-147
Entrez GenePax8 (a.k.a. Pax-8)
- Promoter CMV
/ Fusion Protein
- Eng (N-terminal repressor domain of the drosophila engrailed protein) (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH Reverse (Common Sequencing Primers)
Terms and Licenses
There is a BamHI site in between the Eng and PD coding sequences.
Drosophila Engrailed (Eng)contains H22P and L158P compared to closest reference sequence (AAA65478.1). Depositor states that these mutation should not affect function.
Mouse Pax8 contains a P2A mutation. Depositor states that this mutation should not affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA3-EngPD was a gift from Daniel Christophe (Addgene plasmid # 64039 ; http://n2t.net/addgene:64039 ; RRID:Addgene_64039)
For your References section:Human Thyroid Oxidases genes promoter activity in thyrocytes does not appear to be functionally dependent on Thyroid Transcription Factor-1 or Pax8. Christophe-Hobertus C, Christophe D. Mol Cell Endocrinol. 2007 Jan 29;264(1-2):157-63. Epub 2006 Dec 19. 10.1016/j.mce.2006.11.005 PubMed 17182173