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pcDNA3-EngPD
(Plasmid #64039)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 64039 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5446
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Pax8 paired domain
  • Alt name
    paired box 8
  • Species
    M. musculus (mouse)
  • Mutation
    paired domain, aa 1-147
  • Entrez Gene
    Pax8 (a.k.a. Pax-8)
  • Promoter CMV
  • Tag / Fusion Protein
    • Eng (N-terminal repressor domain of the drosophila engrailed protein) (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH Reverse
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

There is a BamHI site in between the Eng and PD coding sequences.

Drosophila Engrailed (Eng)contains H22P and L158P compared to closest reference sequence (AAA65478.1). Depositor states that these mutation should not affect function.

Mouse Pax8 contains a P2A mutation. Depositor states that this mutation should not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-EngPD was a gift from Daniel Christophe (Addgene plasmid # 64039 ; http://n2t.net/addgene:64039 ; RRID:Addgene_64039)
  • For your References section:

    Human Thyroid Oxidases genes promoter activity in thyrocytes does not appear to be functionally dependent on Thyroid Transcription Factor-1 or Pax8. Christophe-Hobertus C, Christophe D. Mol Cell Endocrinol. 2007 Jan 29;264(1-2):157-63. Epub 2006 Dec 19. 10.1016/j.mce.2006.11.005 PubMed 17182173