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pLH-nmsgRNA1.1 Notes

Addgene Diagnostic Digest

This gel depicts the NEB 1kb ladder and a digestion of the plasmid
64115 with the following enzymes: EcoRI-HF single digest and EcoRI-HF and KpnI-HF double digest.

We expect the linearized plasmid to run at ~8.3kb and the double digest to give 2 bands of ~1.9kb and 6.4kb.

We see the expected result.