ROZA YF
(Plasmid #64197)

• Purpose: Fluorescent Reporter for ZAP-70 tyrosine kinase activity-control YF mutant
• Depositing Lab: Annemarie Lellouch
• Publication: Randriamampita et al PLoS One. 2008 Jan 30;3(1):e1521. doi: 10.1371/journal.pone.0001521.

Backbone

• Vector backbone: pCDNA 3.1+
• Backbone manufacturer: invitrogen
• Backbone size w/o insert (bp): 5445
• Total vector size (bp): 1908
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ROZA YF
• Species: Synthetic
• Insert Size (bp): 1908
• Mutation: tyrosine phosphorylatin site mutated to phenylalanine
• GenBank ID: EU035753
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: Sp6

Resource Information

• A portion of this plasmid was derived from a plasmid made by: This construct includes monomeric variants of CFP and YFP that were obtained from RY Tsien UC San Diego, USA (see reference below)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Reference for monomeric CFP and YFP fluorescent proteins:
Zacharias DA, Violin JD, Newton AC, Tsien RY (2002). Partitioning of lipid-modified monomeric GFPs into membrane microdomains of live cells. Science 296: 913-916.