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(Plasmid #64294)


Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 64294 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 4000
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy


  • Gene/Insert name
  • Species
  • Insert Size (bp)
  • Promoter T7
  • Tags / Fusion Proteins
    • FlAsH (C terminal on insert)
    • 6His (C terminal on insert)

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer pTF 5′-CTCGAAAATAATAAAGGGAAAATCAG-3′
  • 3′ sequencing primer pTR 5′-TGGTAGTGTGGGGACTC-3′
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

Amino acid sequence: mkktaiaiavalagfatvaqaapndntFyLgLkMgIsqyhdtgfinnngpthenqLgAgAfGgYqVnpyVgLeLgVdVlgrmpykgsvengaykaqgVqLtAkLgYpitddVdVyLrLgImVwradtksnvygknhdtgvsPvFaGgVeYaItpeIaTrLeYqWtnnigdYPYDVPDYAtrpdnWmVsLgAsYrfgCCPGCCHHHHHH

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJexpress414_OmpA171_H2467 was a gift from Timothy Whitehead (Addgene plasmid # 64294 ; ; RRID:Addgene_64294)
  • For your References section:

    Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Stapleton JA, Whitehead TA, Nanda V. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836. 10.1073/pnas.1501836112 PubMed 26199411