PurposeConservative version of OmpA Redesign 4 with G125L mutation
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||64298||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4000
Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
Growth Strain(s)XL1 Blue
Copy numberHigh Copy
Insert Size (bp)615
- Promoter T7
/ Fusion Proteins
- FlAsH (C terminal on insert)
- 6His (C terminal on insert)
- Cloning method Unknown
- 5′ sequencing primer pTF 5′-CTCGAAAATAATAAAGGGAAAATCAG-3′
- 3′ sequencing primer pTR 5′-TGGTAGTGTGGGGACTC-3′ (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pJexpress414_OmpA171_R4cons_G125L was a gift from Timothy Whitehead (Addgene plasmid # 64298 ; http://n2t.net/addgene:64298 ; RRID:Addgene_64298)
For your References section:Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Stapleton JA, Whitehead TA, Nanda V. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836. 10.1073/pnas.1501836112 PubMed 26199411