Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Addgene is open for ordering and depositing; find up-to-date details here. To learn more about how we are supporting COVID-19 research and to find related plasmids, check out our COVID-19 and Coronavirus Plasmids & Resources page.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

(Plasmid #64313)


Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 64313 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 4000
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy


  • Gene/Insert name
  • Species
  • Insert Size (bp)
  • Promoter T7
  • Tags / Fusion Proteins
    • FlAsH (C terminal on insert)
    • 6His (C terminal on insert)

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer pTF 5′-CTCGAAAATAATAAAGGGAAAATCAG-3′
  • 3′ sequencing primer pTR 5′-TGGTAGTGTGGGGACTC-3′
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

Amino acid sequence: mkktaiaiavalagfatvaqaapndntWyTgAkLgWsqyhdtgfinnngpthenqLgAgAfGgYqVnpyVgFeMgYdWlgrmpykgsvengaykaqgVqLtAkLgYpitddLdIyTrLgGmVwradtksnvygknhdtgvsPvFaGgVeYaItpeIaMrLeTqHtnnigdYPYDVPDYAtrpdnWmVsLgAsYrfgCCPGCCHHHHHH

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJexpress414_OmpA171_H-78 was a gift from Timothy Whitehead (Addgene plasmid # 64313 ; ; RRID:Addgene_64313)
  • For your References section:

    Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Stapleton JA, Whitehead TA, Nanda V. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836. 10.1073/pnas.1501836112 PubMed 26199411