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8024 GFP_Kras_G12C_2A_puro
(Plasmid #64373)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 64373 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    7432 Bicistronic_GFP_ires_puro
  • Backbone manufacturer
    Theodora Agalioti
  • Backbone size w/o insert (bp) 7432
  • Total vector size (bp) 8047
  • Modifications to backbone
    AgeI, HpaI
  • Vector type
    Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mouse Kras2A
  • Alt name
    Kras
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1356
  • Mutation
    changed glycine 12 to cysteine
  • Entrez Gene
    Kras (a.k.a. AI929937, K-Ras, K-Ras 2, K-ras, Ki-ras, Kras-2, Kras2, c-K-ras, c-Ki-ras, p21B, ras)
  • Tag / Fusion Protein
    • GFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (not destroyed)
  • 3′ cloning site SmaI (destroyed during cloning)
  • 5′ sequencing primer ccctcactccttctctagg
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

This plasmid was constructed by Theodora Agalioti in G. Stathopoulos Lab in Patras, Greece.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    8024 GFP_Kras_G12C_2A_puro was a gift from Georgios Stathopoulos (Addgene plasmid # 64373 ; http://n2t.net/addgene:64373 ; RRID:Addgene_64373)
  • For your References section:

    Mutant KRAS promotes malignant pleural effusion formation. Agalioti T, Giannou AD, Krontira AC, Kanellakis NI, Kati D, Vreka M, Pepe M, Spella M, Lilis I, Zazara DE, Nikolouli E, Spiropoulou N, Papadakis A, Papadia K, Voulgaridis A, Harokopos V, Stamou P, Meiners S, Eickelberg O, Snyder LA, Antimisiaris SG, Kardamakis D, Psallidas I, Marazioti A, Stathopoulos GT. Nat Commun. 2017 May 16;8:15205. doi: 10.1038/ncomms15205. 10.1038/ncomms15205 PubMed 28508873