pOGGUSPlus
(Plasmid
#64400)
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PurposeOat globulin Glo1 promoter driving expression of GUSPlus
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 64400 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepGGFPR (pSP72)
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Modifications to backboneGFP replaced with e GUSPlus coding region and the nopaline synthase (NOS) terminator region isolated from pCAMBIA 1305.1 (CAMBIA, www.cambia.org).
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Vector typePlant Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameOat globulin Glo1 promoter
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Alt nameGlo1
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SpeciesAvena sativa
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Insert Size (bp)960
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GenBank IDAY795082.1
- Promoter Oat globulin Glo1
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Tag
/ Fusion Protein
- GUSplus (modified Staphylococcus sp. b-glucuronidase) (C terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer T7
- 3′ sequencing primer EGFP-C (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pOGGUSPlus was a gift from Claudia Vickers (Addgene plasmid # 64400 ; http://n2t.net/addgene:64400 ; RRID:Addgene_64400) -
For your References section:
A novel cis-acting element, ESP, contributes to high-level endosperm-specific expression in an oat globulin promoter. Vickers CE, Xue G, Gresshoff PM. Plant Mol Biol. 2006 Sep;62(1-2):195-214. Epub 2006 Aug 17. 10.1007/s11103-006-9014-1 PubMed 16915522