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pSS146
(Plasmid #64770)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 64770 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Cre-EBD78
  • Promoter TDH4
  • Tag / Fusion Protein
    • fused to the human Estrogen Binding Domain (EBD), which makes the nuclear activity of Cre dependent on the hormone b-estradiol (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site NotI (destroyed during cloning)
  • 5′ sequencing primer GPDpro-F
  • 3′ sequencing primer CYC2
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

The discrepancies between the depositor's sequence and Addgene’s sequences have no functional consequences.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSS146 was a gift from Fred van Leeuwen (Addgene plasmid # 64770 ; http://n2t.net/addgene:64770 ; RRID:Addgene_64770)
  • For your References section:

    Recombination-induced tag exchange (RITE) cassette series to monitor protein dynamics in Saccharomyces cerevisiae. Terweij M, van Welsem T, van Deventer S, Verzijlbergen KF, Menendez-Benito V, Ontoso D, San-Segundo P, Neefjes J, van Leeuwen F. G3 (Bethesda). 2013 Aug 7;3(8):1261-72. doi: 10.1534/g3.113.006213. 10.1534/g3.113.006213 PubMed 23708297