pGL3-fugu SLC2A4 promoter
PurposeLuciferase reporter construct under the fugu (Takifugu rubripes) SLC2A4 promoter.
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||65101||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4800
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameSLC2A4 promoter
Alt nameGLUT4 promoter
SpeciesFugu (Takifugu rubripes)
Insert Size (bp)1300
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer RVprimer 3
- 3′ sequencing primer LucNrev (Common Sequencing Primers)
Plasmid is also known as pGL3-trSLC2A4P.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pGL3-fugu SLC2A4 promoter was a gift from Josep Planas (Addgene plasmid # 65101 ; http://n2t.net/addgene:65101 ; RRID:Addgene_65101)
For your References section:Mechanisms regulating GLUT4 transcription in skeletal muscle cells are highly conserved across vertebrates. Marin-Juez R, Diaz M, Morata J, Planas JV. PLoS One. 2013 Nov 18;8(11):e80628. doi: 10.1371/journal.pone.0080628. eCollection 2013. PONE-D-13-30467 [pii] PubMed 24260440