|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||65148||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesS. cerevisiae (budding yeast)
- Cloning method Restriction Enzyme
- 5′ cloning site BsmBI (destroyed during cloning)
- 3′ cloning site BsmBI (destroyed during cloning)
Terms and Licenses
Type 3a parts are used for N-terminal coding sequences. This plasmid encodes for an N-terminal protein degradation tag. For more information, please visit the supplemental information in PMID:25871405.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pYTK041 was a gift from John Dueber (Addgene plasmid # 65148 ; http://n2t.net/addgene:65148 ; RRID:Addgene_65148)
For your References section:A Highly-characterized Yeast Toolkit for Modular, Multi-part Assembly. Lee ME, DeLoache WC, Cervantes B, Dueber JE. ACS Synth Biol. 2015 Apr 14. 10.1021/sb500366v PubMed 25871405