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pOPINB-OTUB1*
(Plasmid #65441)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 65441 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pOPINB
  • Backbone manufacturer
    OPPF-UK
  • Backbone size w/o insert (bp) 5531
  • Total vector size (bp) 6836
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    UbcH5b
  • Species
    H. sapiens (human)
  • Mutation
    Active site cysteine mutated to alanine
  • Entrez Gene
    UBE2D2 (a.k.a. E2(17)KB2, PUBC1, UBC4, UBC4/5, UBCH4, UBCH5B)
  • Promoter T7
  • Tag / Fusion Protein
    • His-3C (N terminal on backbone)

Cloning Information for Gene/Insert 1

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer T7 terminator
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    otubain-1
  • Entrez Gene
    OTUB1 (a.k.a. HSPC263, OTB1, OTU1)

Cloning Information for Gene/Insert 2

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer T7 terminator
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Article Citing this Plasmid
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pOPINB-OTUB1* was a gift from David Komander (Addgene plasmid # 65441 ; http://n2t.net/addgene:65441 ; RRID:Addgene_65441)
  • For your References section:

    Assembly and specific recognition of k29- and k33-linked polyubiquitin. Michel MA, Elliott PR, Swatek KN, Simicek M, Pruneda JN, Wagstaff JL, Freund SM, Komander D. Mol Cell. 2015 Apr 2;58(1):95-109. doi: 10.1016/j.molcel.2015.01.042. Epub 2015 Mar 5. 10.1016/j.molcel.2015.01.042 PubMed 25752577