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pCaSpeR3/ncd-gfp #10
(Plasmid #66082)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 66082 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCaSpeR3
  • Backbone size w/o insert (bp) 7814
  • Modifications to backbone
    Plasmid contains 3.1kb ncd promoter, 2.1kb ncd coding region and 0.7kb GFP
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Medium copy number plasmid; Preferred growth temperature 34-35C
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Ncd kinesin-14 motor protein
  • Alt name
    Ca[nd]
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    5900
  • Mutation
    D699L
  • GenBank ID
    X52814
  • Entrez Gene
    ncd (a.k.a. Dmel_CG7831, CG7831, DmNCD, DmNcd, Dmel\CG7831, KIF C1, KIFC1, NCD, Ncd, ca, ca[nd], cd, l(3)05884, ndc)
  • Promoter ncd promoter (3.1kb upstream region of ncd start site)
  • Tag / Fusion Protein
    • GFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (destroyed during cloning)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pCasper-F (5'-GGGTTTTATTAACTTACAT-3')
  • 3′ sequencing primer Cas3 Rev (5'-AACTCATAATATTAATTAGACG-3')
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Other references describing Ncd-GFP expression:

Endow SA & Komma DJ 1996 J Cell Sci 109, 2429-2442 - PubMed ID: 8923204

Skold HN, Komma DJ & Endow SA 2005 J Cell Sci 118, 1745-1755 - PubMed ID: 15797926

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCaSpeR3/ncd-gfp #10 was a gift from Sharyn Endow (Addgene plasmid # 66082 ; http://n2t.net/addgene:66082 ; RRID:Addgene_66082)
  • For your References section:

    Spindle dynamics during meiosis in Drosophila oocytes. Endow SA, Komma DJ. J Cell Biol. 1997 Jun 16;137(6):1321-36. PubMed 9182665