pCVD450
(Plasmid
#66153)
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Purposecloning of E coli E2348/69 PerA
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 66153 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepACYC184
- Backbone size w/o insert (bp) 4300
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Vector typecloning vector
Growth in Bacteria
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Bacterial Resistance(s)Tetracycline, 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameperA
- Promoter none
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer CAT_int (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
E coli E2348/69 PerA plasmid regulator. perA subclone from pMAR2. ~ 5 kb EcoRI fragment of pMAR2 (GenBank accession number FM180569.1) containing perA, perB and perC cloned into the EcoRI site of pACYC184.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCVD450 was a gift from James Kaper (Addgene plasmid # 66153 ; http://n2t.net/addgene:66153 ; RRID:Addgene_66153) -
For your References section:
Studies on the regulation of virulence gene expression of enteropathogenic Escherichia coli. Gomez OG. University of Maryland, Baltimore. Dissertation (1993). http://hdl.handle.net/10713/1623