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PhyB-mCherry-Tom7
(Plasmid #66571)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 66571 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pRS305
  • Total vector size (bp) 12448
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    Top10
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PhyB
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    2724
  • Mutation
    PhyB portion contains aa's 1-908
  • Entrez Gene
    PHYB (a.k.a. AT2G18790, HY3, MSF3.17, MSF3_17, OOP1, OUT OF PHASE 1, PHYTOCHROME B, phytochrome B)
  • Promoter pADH1
  • Tags / Fusion Proteins
    • mCherry (C terminal on insert)
    • Tom7 (C terminal on insert)

Cloning Information

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PhyB-mCherry-Tom7 was a gift from Orion Weiner (Addgene plasmid # 66571)
  • For your References section:

    Probing Yeast Polarity with Acute, Reversible, Optogenetic Inhibition of Protein Function. Jost AP, Weiner OD. ACS Synth Biol. 2015 Jun 2. 10.1021/acssynbio.5b00053 PubMed 26035630