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pcDNA3.1-StrepHA-SUMO2
(Plasmid #66867)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 66867 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.1(+)
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5428
  • Total vector size (bp) 5823
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SUMO2
  • Alt name
    small ubiquitin-like modifier 2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    450
  • GenBank ID
    AAH08450.1
  • Entrez Gene
    SUMO2 (a.k.a. HSMT3, SMT3B, SMT3H2, SUMO3, Smt3A)
  • Promoter CMV
  • Tags / Fusion Proteins
    • Strep (N terminal on insert)
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMV For
  • 3′ sequencing primer BGH Rev
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-StrepHA-SUMO2 was a gift from Ying Liu (Addgene plasmid # 66867 ; http://n2t.net/addgene:66867 ; RRID:Addgene_66867)
  • For your References section:

    Regulation of SUMO2 target proteins by the proteasome in human cells exposed to replication stress. Bursomanno S, McGouran JF, Kessler BM, Hickson ID, Liu Y. J Proteome Res. 2015 Apr 3;14(4):1687-99. doi: 10.1021/pr500997p. Epub 2015 Mar 19. 10.1021/pr500997p PubMed 25748227